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Literature summary extracted from

  • Tyas, L.; Moon, R.P.; Loetscher, H.; Dunn, B.M.; Kay, J.; Ridley, R.G.; Berry, C.
    Plasmepsins I and II from the malarial parasite Plasmodium falciparum (1998), Adv. Exp. Med. Biol., 436, 407-411.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.23.38 expression in Escherichia coli Plasmodium falciparum
3.4.23.39 expression in Escherichia coli Plasmodium falciparum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.23.38 N'1-[(E)-(2,3-dihydroxyphenyl)methylidene]-N'3-[(Z)-(2,3-dihydroxyphenyl)methylidene]benzene-1,3-dicarbohydrazide
-
 Plasmodium falciparum
3.4.23.38 Ro40-4388
-
 Plasmodium falciparum
3.4.23.38 Ro40-5576
-
 Plasmodium falciparum
3.4.23.39 N'1-[(E)-(2,3-dihydroxyphenyl)methylidene]-N'3-[(Z)-(2,3-dihydroxyphenyl)methylidene]benzene-1,3-dicarbohydrazide
-
 Plasmodium falciparum
3.4.23.39 Ro40-4388
-
 Plasmodium falciparum
3.4.23.39 Ro40-5576
-
 Plasmodium falciparum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.23.38 0.01
-
 Leu-Glu-Arg-Ile-Phe-Phe(NO2)-Ser-Phe pH 4.4, 37°C Plasmodium falciparum
3.4.23.38 0.03
-
 Leu-Glu-Arg-Val-Phe-Phe(NO2)-Ser-Phe pH 4.4, 37°C Plasmodium falciparum
3.4.23.39 0.006
-
 Lys-Pro-Ile-Val-Phe-Phe(NO2)-Arg-Leu pH 4.7, 37°C Plasmodium falciparum
3.4.23.39 0.02
-
 Leu-Glu-Arg-Val-Phe-Phe(NO2)-Ser-Phe pH 4.4, 37°C Plasmodium falciparum
3.4.23.39 0.025
-
 Leu-Glu-Arg-Ile-Phe-Phe(NO2)-Ser-Phe pH 4.4 Plasmodium falciparum
3.4.23.39 0.06
-
 Ala-Leu-Glu-Arg-Thr-Phe-Phe(NO2)-Ser-Phe-Pro-Thr pH 4.7, 37°C Plasmodium falciparum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.23.38 Hemoglobin + H2O Plasmodium falciparum during the infection of erythrocytes by the human malaria parasite Plasmodium falciparum, up to 80% of the host cell hemoglobin is degraded to provide amino acids for parasite nutrition. Plasmepsin I and plasmepsin II are believed to be responsible for initiating the catabolic process ?
-
 ?
3.4.23.39 Hemoglobin + H2O Plasmodium falciparum during the infection of erythrocytes by the human malaria parasite Plasmodium falciparum, up to 80% of the host cell hemoglobin is degraded to provide amino acids for parasite nutrition. Plasmepsin I and plasmepsin II are believed to be responsible for initiating the catabolic process ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.23.38 Plasmodium falciparum
-
-
-
3.4.23.39 Plasmodium falciparum
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.23.38 Hemoglobin + H2O during the infection of erythrocytes by the human malaria parasite Plasmodium falciparum, up to 80% of the host cell hemoglobin is degraded to provide amino acids for parasite nutrition. Plasmepsin I and plasmepsin II are believed to be responsible for initiating the catabolic process Plasmodium falciparum ?
-
 ?
3.4.23.38 Leu-Glu-Arg-Ile-Phe-Phe(NO2)-Ser-Phe + H2O
-
 Plasmodium falciparum Leu-Glu-Arg-Ile-Phe + Phe(NO2)-Ser-Phe
-
 ?
3.4.23.38 Leu-Glu-Arg-Val-Phe-Phe(NO2)-Ser-Phe + H2O
-
 Plasmodium falciparum Leu-Glu-Arg-Val-Phe + Phe(NO2)-Ser-Phe
-
 ?
3.4.23.38 additional information plasmepsin I is 5 to 9times less effective than plasmepsin II at cleaving substrates which mimic the proposed initial cleavage site in the alpha-chain of hemoglobin Plasmodium falciparum ?
-
 ?
3.4.23.39 Ala-Leu-Glu-Arg-Thr-Phe-Phe(NO2)-Ser-Phe-Pro-Thr + H2O
-
 Plasmodium falciparum Ala-Leu-Glu-Arg-Thr-Phe + Phe(NO2)-Ser-Phe-Pro-Thr
-
 ?
3.4.23.39 Hemoglobin + H2O during the infection of erythrocytes by the human malaria parasite Plasmodium falciparum, up to 80% of the host cell hemoglobin is degraded to provide amino acids for parasite nutrition. Plasmepsin I and plasmepsin II are believed to be responsible for initiating the catabolic process Plasmodium falciparum ?
-
 ?
3.4.23.39 Leu-Glu-Arg-Ile-Phe-Phe(NO2)-Ser-Phe + H2O
-
 Plasmodium falciparum Leu-Glu-Arg-Ile-Phe + Phe(NO2)-Ser-Phe
-
 ?
3.4.23.39 Leu-Glu-Arg-Val-Phe-Phe(NO2)-Ser-Phe + H2O
-
 Plasmodium falciparum Leu-Glu-Arg-Val-Phe + Phe(NO2)-Ser-Phe
-
 ?
3.4.23.39 Lys-Pro-Ile-Lys-Phe-Phe(NO2)-Arg-Leu + H2O
-
 Plasmodium falciparum Lys-Pro-Ile-Lys-Phe + Phe(NO2)-Arg-Leu
-
 ?
3.4.23.39 Lys-Pro-Ile-Val-Phe-Phe(NO2)-Arg-Leu + H2O
-
 Plasmodium falciparum Lys-Pro-Ile-Val-Phe + Phe(NO2)-Arg-Leu
-
 ?
3.4.23.39 additional information the enzyme has a preference for a small hydrophobic residue in the P3 position of the substrate. The S2 and S3 subsites of plasmepsin II are hydrophobic in nature and on this basis the hemoglobin-related substrate, with Arg and Thr in the P3 and P2 positions respectively may not represent the optimal substrate Plasmodium falciparum ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.23.38 1.3
-
 Leu-Glu-Arg-Ile-Phe-Phe(NO2)-Ser-Phe pH 4.4, 37°C Plasmodium falciparum
3.4.23.38 1.6
-
 Leu-Glu-Arg-Val-Phe-Phe(NO2)-Ser-Phe pH 4.4, 37°C Plasmodium falciparum
3.4.23.39 0.04
-
 Lys-Pro-Ile-Lys-Phe-Phe(NO2)-Arg-Leu pH 4.7, 37°C Plasmodium falciparum
3.4.23.39 3.6
-
 Lys-Pro-Ile-Val-Phe-Phe(NO2)-Arg-Leu pH 4.7, 37°C Plasmodium falciparum
3.4.23.39 6
-
 Ala-Leu-Glu-Arg-Thr-Phe-Phe(NO2)-Ser-Phe-Pro-Thr pH 4.7, 37°C Plasmodium falciparum
3.4.23.39 9
-
 Leu-Glu-Arg-Val-Phe-Phe(NO2)-Ser-Phe pH 4.4, 37°C Plasmodium falciparum
3.4.23.39 15
-
 Leu-Glu-Arg-Ile-Phe-Phe(NO2)-Ser-Phe pH 4.4 Plasmodium falciparum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.23.38 5
-
-
 Plasmodium falciparum
3.4.23.39 5
-
-
 Plasmodium falciparum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.23.38 3 7 active over the range Plasmodium falciparum
3.4.23.39 3.5 6.5 active over the range Plasmodium falciparum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.23.38 0.000004
-
 Ro40-4388 pH 4.4, 37°C Plasmodium falciparum
3.4.23.38 0.000006
-
 Ro40-5576 pH 4.4 Plasmodium falciparum
3.4.23.38 0.0003
-
 N'1-[(E)-(2,3-dihydroxyphenyl)methylidene]-N'3-[(Z)-(2,3-dihydroxyphenyl)methylidene]benzene-1,3-dicarbohydrazide pH 4.4 Plasmodium falciparum
3.4.23.39 0.00025
-
 Ro40-5576 pH 4.4, 37°C Plasmodium falciparum
3.4.23.39 0.0005
-
 Ro40-4388 pH 4.4, 37°C Plasmodium falciparum
3.4.23.39 0.004
-
 N'1-[(E)-(2,3-dihydroxyphenyl)methylidene]-N'3-[(Z)-(2,3-dihydroxyphenyl)methylidene]benzene-1,3-dicarbohydrazide pH 4.4, 37°C Plasmodium falciparum